All functions

add_PTM_positions()

Add a column describing the position(s) of the PTM(s) with respect to the protein

add_peptide_positions()

Add a column describing the position(s) of the peptide sequence with respect to the protein

add_site_sequence()

Add a column with amino acid sequence around a PTM

append_fasta()

Append sequences to end of a FASTA

center_normalise_to_ref()

Center-median normalise the expression matrix in an MSnSet using medians from a reference dataset

check_uniprot_release()

Check the current UniProt release

count_features_per_protein()

Count the number of features (rows) per protein

dep_gene2cat

GO terms assigned to proteins in DEP results

dep_results

Results table from DEP package

download_ccp_crap()

Download CCP cRAP FASTA file

estimate_go_overrep()

Estimate effect size of GO over-representation

estimate_incorporation()

Estimate SILAC (2-plex) incorporation rate from PD output

filter_TMT_PSMs() filter_TMT_PSMs()

Filter a PSM-level MSnSet to remove low quality PSMs

get_ancestor_go()

Get all ancestor GO terms

get_cat_palette()

Generate a colour-blind friendly palette for categorical colour encoding

get_ccp_crap()

Get the UniProt sequences in CCP cRAP

get_enriched_go()

GO term enrichment using goseq

get_incorporation()

Determine SILAC (2-plex) incorporation rate given intensity values for Light and Heavy

get_medians()

Extract the expression matrix column medians from an MSnSet

get_notch_per_protein()

Summarise the number of sub-notch PSM intensities per protein

get_parsimony_pep2prot()

Identify the consensus peptide to master protein mapping across multiple runs

get_psm_metrics()

Summarise percentage of tag intensities below a given threshold and missing

get_psm_silac_mod_regex()

Get a pre-defined regex for a SILAC modification in PSM format

get_ratio()

Get ratio of two numbers

get_sequence()

Get the amino acid sequence around a PTM

lfq_oops_rnase_pep

PeptideGroups PD output for LFQ of RNase +/- OOPS experiment

lfq_oops_rnase_psm

PSM PD output for LFQ of RNase +/- OOPS experiment

make_fasta()

Make a FASTA using UniProt accessions

parse_PTM_scores()

Parse the PTM probabilities and add new columns with PTM information

parse_features()

Parse Proteome Discoverer output

pep_oops_1

PeptideGroups PD output for SILAC labelled U-2 OS cells

pep_oops_2

PeptideGroups PD output for SILAC labelled U-2 OS cells

pep_oops_3

PeptideGroups PD output for SILAC labelled U-2 OS cells

pep_oops_4

PeptideGroups PD output for SILAC labelled U-2 OS cells

pep_silac_p0

PeptideGroups PD output for SILAC labelled MOLM-13 cells

pep_silac_p4

PeptideGroups PD output for SILAC labelled MOLM-13 cells

plot_TMT_notch()

Plot histograms for TMT tag intensities including notch annotation

plot_below_notch_per_prot()

Plot the number of sub-notch PSM intensities per protein

plot_fraction_below_notch_per_prot()

Plot the fraction of sub-notch PSM intensities per protein

plot_go()

Plot selected GO terms

plot_incorporation()

Plot annotated histogram of incorporation values

plot_missing_SN()

Plot the missing values vs signal:noise

plot_missing_SN_per_sample()

Plot the missing values vs signal:noise for each sample

plot_quant()

Plot distributions for feature intensities per sample.

psm_oops_1

PSM PD output for SILAC labelled U-2 OS cells

psm_oops_2

PSM PD output for SILAC labelled U-2 OS cells

psm_oops_3

PSM PD output for SILAC labelled U-2 OS cells

psm_oops_4

PSM PD output for SILAC labelled U-2 OS cells

psm_silac_p4

PSMs PD output for SILAC labelled MOLM-13 cells

psm_tmt_phospho

PSM-level PD output for phospho TMT10-plex data

psm_tmt_total

PSM-level PD output for total proteome TMT10-plex data

psm_to_peptide_style_modifications()

Transformes PSM-level Modifications annotation style to peptide-level style

remove_dots()

Remove duplicated full stops

remove_redundant_go()

Remove redundant GO terms

remove_silac_modifications()

Remove SILAC labels from modifications

remove_x()

Remove leading X

restrict_features_per_protein()

Remove features which are assigned to a protein with too few supporting features in total

silac_psm_seq_int()

Extract peptide sequencing and interference information from SILAC PSM-level output

sub_crap()

Insert cRAP numbers into a character vector

theme_camprot()

ggplot theme for package

theme_csd()

Charlotte's ggplot2 theme

update_average_sn()

Adds new feature describing the average reporter Signal/Noise ratio.