center_normalise_to_ref.Rd
Center-median normalisation is a simple normalisation method
that is appropriate for relative abundance proteomics such as isobaric tagging.
This can be achieved with MSnbase::normalise(method='diff.median')
. However,
for some experimental designs, the normalisation should be against the medians
in another dataset. For example, for PTM studies, one may wish to isobaric
tag samples, pool, and then PTM-enriched, with the enriched sample quantified
in a separate run to the non-enriched (total) sample. In this case, it may make
more sense to center-median normalise the PTM-enriched samples using the median
from the total samaples.
center_normalise_to_ref(
obj,
medians,
center_to_zero = FALSE,
on_log_scale = FALSE
)
MSnSet
. Contains PSMs.
vector, numeric
. Sample medians from reference dataset
logical
. Centre the data range on zero.
If FALSE, normalisation retains original data range.
logical
. Input data is log-transformed
Returns an MSnSet
with the expression matrix column center-median
normalised